SAE0095

Agrin Human

Recombinant, cell culture tested, expressed in HEK 293 cells

• Synonym(s): AGRN, Proteoglycan
• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: lyophilized powder
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in HEK 293 cells

Properties

• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: attachment assay, measured by the ability of the immobilized agrin to support the adhesion of PC12 cells
• technique(s): cell culture | mammalian: suitable
• suitability: endotoxin tested
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Agrin C-terminal human recombinant is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 87 kDa (amino acids Ala1260-Pro2045, with an N-terminal His tag). The DTT-reduced protein migrates as a ∼100 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher bioactivity of the protein.

Biochem/physiol Actions

Agrin is a large extracellular heparan sulfate proteoglycan that is involved in the formation of the neuromuscular junction.
Agrin triggers the aggregation of acetylcholine receptors via the muscle-specific kinase (MuSK) and the low-density lipoprotein receptor-related protein 4 (Lrp4) receptor complex.
Agrin is required for the full regenerative capacity of neonatal mouse hearts.
Recombinant agrin has been shown in vitro to promote cardiomyocyte proliferation, as studied in mouse-induced and human-induced pluripotent stem cells. Recombinant agrin has been shown in vivo to promote cardiac regeneration, after induction of myocardial infarction in adult mice.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 2