The PAD4, SAE0086, is not routinely tested by gel electrophoresis. Product development data reveals a molecular weight of is 74.1 kDa.
SAE0086
Peptidyl Arginine Deiminase Type 4
human recombinant, expressed in E. coli, aqueous solution
Synonym(s):
Peptidyl Arginine Deiminase Type 4, PAD4, PADI4, Protein arginine iminohydrolase 4
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| Size/SKU | Availability | Price |
|---|---|---|
25 units | Please contact Customer Service for Availability | 14 700,00 CZK |
About This Item
EC Number:
3.5.3.15
NACRES:
NA.54
UNSPSC Code:
12352204
Form:
aqueous solution
Assay:
≥90% (SDS-PAGE)
Mol wt:
74 kDa
14 700,00 CZK
Please contact Customer Service for Availability
Quality Segment
assay
≥90% (SDS-PAGE)
form
aqueous solution
specific activity
≥200 units/mg protein
mol wt
74 kDa
UniProt accession no.
application(s)
genomic analysis
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... PADI4(23569)
General description
Peptidyl Arginine Deiminases (PADs) perform post-translational deiminations of proteins. PADs are calcium-dependent enzymes that catalyze the conversion of L-arginine residues to L-citrulline. The catalyzed reaction is as follows:
Ca2+ Protein--[L-arginine] + H2O ––––> Protein--[L-citrulline]
There are five mammalian PADs sub-types, which differ in substrate specificity and tissue distribution. PAD enzymes are highly homologous, with 50–60% sequence similarity.1 PADs play important roles in gene regulation by citrullination of arginine residues on histones H3, H2A, and H4. Overexpression of PADs has been found in conditions such as rheumatoid arthritis, Alzheimer′s disease, multiple sclerosis, lupus,Parkinson′s disease, and cancer.2,3
PAD4 is widely expressed in the immune system with expression pathology in various tumor and cancer cell lines.5 In cells, PAD4 can be found in the cytoplasm and nucleus. PAD4 activity has been correlated to the development and progress of Rheumatoid Arthritis (RA).2,5 A study in mice has indicated a mechanism for the development of RA by anticitrullin autoimmunity.6
This recombinant human PAD4 is expressed in Escherichia coli as a fusion protein. The fusion partner is removed by HRV3C protease digestion, followed by chromatographic purification.
Ca2+ Protein--[L-arginine] + H2O ––––> Protein--[L-citrulline]
There are five mammalian PADs sub-types, which differ in substrate specificity and tissue distribution. PAD enzymes are highly homologous, with 50–60% sequence similarity.1 PADs play important roles in gene regulation by citrullination of arginine residues on histones H3, H2A, and H4. Overexpression of PADs has been found in conditions such as rheumatoid arthritis, Alzheimer′s disease, multiple sclerosis, lupus,Parkinson′s disease, and cancer.2,3
PAD4 is widely expressed in the immune system with expression pathology in various tumor and cancer cell lines.5 In cells, PAD4 can be found in the cytoplasm and nucleus. PAD4 activity has been correlated to the development and progress of Rheumatoid Arthritis (RA).2,5 A study in mice has indicated a mechanism for the development of RA by anticitrullin autoimmunity.6
This recombinant human PAD4 is expressed in Escherichia coli as a fusion protein. The fusion partner is removed by HRV3C protease digestion, followed by chromatographic purification.
Biochem/physiol Actions
Calcium is required for peptidylarginine deiminase activity in vitro.
Physical form
Supplied as an aqueous solution in 10 mM Tris (pH 7.5), 500 mM NaCl, 1 mM EDTA, and 1 mM DTT.
Other Notes
One unit will produce 1 μmole of N-a-benzoylcitrulline ethyl ester from BAEE per hour at 37 °C at pH 7.7.
1 of 1
This Item | |||
|---|---|---|---|
| description human recombinant, expressed in E. coli, aqueous solution | description IgG fraction of antiserum, buffered aqueous solution | description clone 4D8, purified immunoglobulin, buffered aqueous solution | description recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution |
| assay ≥90% (SDS-PAGE) | assay - | assay - | assay ≥70% (SDS-PAGE) |
| form aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous glycerol solution |
| mol wt 74 kDa | mol wt antigen 67 kDa | mol wt - | mol wt ~99 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
| Gene Information human ... PADI4(23569) | Gene Information human ... PADI4(23569) | Gene Information human ... PADI4(23569) | Gene Information mouse ... Padi2(18600) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
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