SAE0069

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

• Synonym(s): CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB
• UNSPSC Code: 12352200
• NACRES: NA.32
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder

Properties

• Product Name: Cholera Toxin B subunit, recombinant, expressed in HEK 293 cells, subunit A free
• recombinant: expressed in HEK 293 cells
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• specific activity: (Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)
• mol wt: pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing), calculated mol wt 13 kDa, observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)
• solubility: H₂O: soluble 11 mg/mL
• UniProt accession no.: P01556
• storage temp.: −20°C

Description

General description

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).

The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.

Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.

Application

Cholera Toxin B subunit has been used:

• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Preparation Note

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable