SAB4200780

Anti-Human IgM (μ-chain specific) antibody, Mouse monoclonal

clone MB-11, purified from hybridoma cell culture

• Synonym(s): Anti-Human immunoglobulin M
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 300
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: MB-11, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating; immunoblotting: suitable
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgM protein.

Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocyte membranes. IgM is suggested to act as the first line of defense during microbial infections. IgM has a pentameric structure, in which monomers are linking together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.

Specificity

Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal specifically recognizes m chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, k, l, and g chains.

Immunogen

Purified human IgM

Application

The antibody is recommended to use in various immunological techniques, including ELISA and Immunoblot.

Biochem/physiol Actions

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable