S9446

Sphingosine Kinase 1 human

aqueous glycerol solution

• Synonym(s): SPHK 1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 2.7.1.91 (BRENDA, IUBMB)
• MDL number: MFCD09265456
• Specific activity: ≥10 units/μg
• Recombinant: expressed in baculovirus infected insect cells

Properties

• recombinant: expressed in baculovirus infected insect cells
• Quality Segment: 200
• form: solution
• specific activity: ≥10 units/μg
• mol wt: 47 kDa
• UniProt accession no.: Q9NYA1
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SPHK1(8877)

Description

General description

Sphingosine 1-phosphate-induced release of TIMP-2 from vascular smooth muscle cells inhibits angiogenesis. [1]

Application

Sphingosine kinase 1 has been used in a study to assess the modulation of cellular S1P levels. [2] Sphingosine kinase 1 has also been used in a study to investigate how FTY720 normalizes hyperglycemia. [3]

Biochem/physiol Actions

Converts sphingosine to sphingosine-1-phosphate. SPHK1 shares two large conserved regions with SPHK2. While SPHK1 is found only in the cytosol, SPHK2 is localized in both the cytosol and nuclei.

Physical form

Supplied in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween 20, 50% glycerol, 3 mM DTT.

aqueous glycerol solution

Other Notes

One unit will produce 1.0 pmole of sphingosine-1-phosphate from ATP and D-erythro-sphingosine per minute at pH 7.4, 37 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)