S7897
Sarcosine Oxidase from Bacillus sp.
lyophilized powder, 25-50 units/mg solid
Synonym(s):
Sarcosine: oxygen oxidoreductase (demethylating)
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| Size/SKU | Availability | Price |
|---|---|---|
1000 units | Please contact Customer Service for Availability | CZK 16,100.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-850-5
MDL number:
Specific activity:
25-50 units/mg solid
CZK 16,100.00
Please contact Customer Service for Availability
form
lyophilized powder
Quality Segment
specific activity
25-50 units/mg solid
mol wt
44 kDa
storage temp.
−20°C
General description
Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline. [1]
Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD.
Application
Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. [2] Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins. [3]
Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV. It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor.[4]
Physical form
No stabilizers added
Other Notes
One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 25-50 units/mg solid | specific activity ≥0.45 units/mg protein | specific activity 8-20 units/mg solid | specific activity ≥3 units/mg solid, ≥40 U/mg |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized solid |
| mol wt 44 kDa | mol wt ~88 kDa by electrophoresis | mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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