S6311

Anti-phospho-S6-Kinase (pThr³⁸⁹) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Anti-phospho-p70^S6K (pThr³⁸⁹)
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD02097170
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB CL
• Citations: 8

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: mouse, rat, human
• technique(s): western blot (chemiluminescent): 1:1,000 using serum-treated NIH3T3 cell extract, or insulin-treated HeLa, COS, and C6 cell extracts
• UniProt accession no.: P23443
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer389)
• Gene Information: human ... RPS6KB1(6198) ; mouse ... Rps6kb1(72508) ; rat ... Rps6kb1(83840)

Description

General description

p70 S6 kinase or p70S6K is a protein that belongs to Ser/Thr protein kinase family. It has a crucial role in cell growth and G1 cell cycle progression. It also has a pivotal role in phosphorylating eEF2k at a conserved serine residue and hence regulating the protein synthesis. Rabbit anti-phospho-(p70S6K) antibody can identify p70 S6 kinase and p85 S6 kinase when phosphorylated at (Thr³⁸⁹). Anti-phospho-S6-kinase (pThr³⁸⁹) antibody reacts specifically with p70 S6 kinase of rat, mouse and human. This product can also detect few phosphorylated isoforms of Protein Kinase C when present at high level in cells.

Immunogen

A synthetic phospho-(Thr³⁸⁹) peptide corresponding to residues around (Thr³⁸⁹) of human p70 S6 kinase, coupled to KLH.

Application

Anti-phospho-S6-kinase (pThr³⁸⁹) antibody can be used in western blotting (chemiluminescent) (diluted 1:1000) using serum-treated NIH3T3 cell extract or insulin-treated HeLa, COS, and C6 cell extracts.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable