S6147

Sephadex^® G-100

DNA grade, BioReagent, Molecular Biology, medium

• CAS Number: 9050-94-6
• UNSPSC Code: 23151817
• NACRES: NA.52
• MDL number: MFCD00002744

Properties

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioReagent
• form: beads
• technique(s): buffer exchange: suitable
• matrix active group: phase
• swelling: 1 g swells to 9-11 mL
• bead diameter: 40-120 μm
• pore size: exclusion limit (25 bp dsDNA; (rA)27)
• pH: 2-10
• application(s): life science and biopharma
• compatibility: Cytiva
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp

Description

General description

Sephadex^® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex^® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Features and Benefits

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)