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S4760

Sigma-Aldrich

N-Succinyl-Ala-Ala-Ala-p-nitroanilide

elastase substrate, chromogenic, ≥98% (HPLC), powder

Synonym(s):

N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide, N-Succinyl-tri-L-alanine 4-nitroanilide

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About This Item

Empirical Formula (Hill Notation):
C19H25N5O8
CAS Number:
52299-14-6
Molecular Weight:
451.43
Beilstein:
2928351
EC Number:
257-823-5
MDL number:
MFCD00036774
UNSPSC Code:
12352204
PubChem Substance ID:
24899617
NACRES:
NA.32

product name

N-Succinyl-Ala-Ala-Ala-p-nitroanilide, elastase substrate

Assay

≥98% (HPLC)

form

powder

solubility

DMF: 25 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

SMILES string

C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1

InChI key

GVUGADOWXGKRAE-SRVKXCTJSA-N

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General description

N-Succinyl-Ala-Ala-Ala-p-nitroanilide is a chromogenic substrate for chymotrypsin-like serine protease, pancreatic elastase and serine endopeptidase.

Application

N-Succinyl-Ala-Ala-Ala-p-nitroanilide has been used as a substrate
  • for screening elastase activity in lyophilized earthworm fibrinolytic enzymes
  • for quantifying elastase activity in sheep milk
  • in screening honeybee inhibitors in serine protease inhibition assay

Substrates

Elastase substrate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Ogawa et al.
Research communications in chemical pathology and pharmacology, 55(2), 271-274 (1987-02-01)
A human urinary trypsin inhibitor preparation, MR-20, strongly inhibited granulocyte elastase activity. The concentration of MR-20 causing 50% inhibition of the hydrolysis of Suc(Ala) 3pNA by granulocyte elastase was estimated to be 6.4 X 10(-8) M (11 U/ml).
William R Swindell et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Tetrahexyldecyl Ascorbate (THDC) is an L-ascorbic acid precursor with improved stability and ability to penetrate the epidermis. The stability and transdermal penetration of THDC, however, may be compromised by the oxidant-rich environment of human skin. In this study, we show
Vinu S Siva et al.
Fish & shellfish immunology, 59, 1-8 (2016-11-05)
Serine protease inhibitors (SPIs) play a crucial role in regulation of both host and bacterial serine protease. They are classified into several protein families, where Kazal-type inhibitors are one of families with multi-domain. In the present study, the polymorphism of
Hu Wan et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 165(1), 36-41 (2013-03-19)
Spider-derived Kunitz-type serine protease inhibitors have been shown to exhibit plasmin and elastase inhibition activity and potassium channel blocking activity, but thus far, no additional roles for spider-derived chymotrypsin inhibitors have been elucidated. In this study, a spider (Araneus ventricosus)
T Fera et al.
The American review of respiratory disease, 133(4), 568-573 (1986-04-01)
In order to determine the acute effect of smoking on elastase in bronchoalveolar lavage fluid (BAL), we obtained BAL from 30 smokers twice, the first before smoking (after 8 h of abstinence) and the second 2 min to 1 h
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