S3917

Single-strand Binding Protein from Escherichia coli

>95% (SDS-PAGE), Molecular Biology, Single strand binding protein to enhance PCR specificity

• Synonym(s): SSB
• UNSPSC Code: 12352202
• NACRES: NA.52
• MDL number: MFCD00213047
• Form: liquid
• Assay: >95% (SDS-PAGE)

Properties

• grade: Molecular Biology
• Quality Segment: 200
• assay: >95% (SDS-PAGE)
• form: liquid
• color: colorless
• foreign activity: DNase and RNase, none detected
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism.

Application

Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites.

Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.

Biochem/physiol Actions

Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H318,H410,EUH430
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1
• Storage Class: 10 - Combustible liquids
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501