The suitability of this serum product has not been tested following incubation at 37°C. Fetal Bovine Serum is typically heat-inactivated by incubating at 56°C for 30 minutes.
This treatment inactivates the complement system, as well as other undetermined constituents that impact cell growth in culture. Culture medium containing heat-inactivated sera are often supplemented with specific cytokines and/or hormones to assure cell health. Some more robust cell lines may better tolerate medium that has been treated for 72 hours at 37°C, however general cell culture protocols typically require medium refreshment or replacement every 2 – 3 days to maintain a healthy cell population.
S0615
FBS Superior
Supplemented FBS, EU approved
FBS Superior
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
FBS, FCS, Fetal Bovine Sera, Fetal Bovine Serum, Supplemented FCS
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Please contact Customer Service for Availability | CZK 3,840.00 |
500 mL | Please contact Customer Service for Availability | CZK 15,100.00 |
About This Item
NACRES:
NA.71
UNSPSC Code:
12352207
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
CZK 3,840.00
Please contact Customer Service for Availability
biological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Select
quality
EU approved
composition
hemoglobin, <50 mg/dL
origin
non-USA origin
impurities
≤25 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
Application
FBS Superior has been used:
- as a component of differentiation medium, growth medium, induction medium, and Dulbecco′s modified Eagle medium (DMEM) for murine thermogenic adipocytes culture[3]
- as a component of the culture medium along with DMEM high glucose (HG) for cell viability check[4]
- as a component of minimum essential medium Eagle (MEM) to cultivate Caco-2 human colon adenocarcinoma cells[5]
Biochem/physiol Actions
Fetal bovine serum(FBS) Superior is a South American source of FBS supplemented with various growth-promoting compounds to allow for a more consistent product. This supplementation can reduce or eliminate the need to test multiple batches on specific cell lines. FBS Superior can be used just like regular FBS, delivers nutrients, growth, and attachment factors, and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.
Fetal bovine serum (FBS) comprises growth factors (GFs) and other elements that are required for the expansion, maintenance, attachment, and proliferation of adipose-derived stromal cells (ASC) in vitro.[1]
1 of 1
This Item | |||
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility γ-irradiated, sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| origin non-USA origin | origin USA origin | origin USA origin | origin non-USA origin |
| composition hemoglobin, <50 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤25 mg/dL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities ≤25 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Wie lange ist dieses FBS als 10%iges Supplement in RPMI 1640-Medium bei einer Temperatur von 37+/-1°C stabil? Kann RPMI 1640-Medium mit 10% von diesem FBS nach 72+/2h bei 37+/1°C noch für Zellkulturanwendungen verwendet werden?
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Est ce que le FBS superior S06115 doit être décomplémenté?
1 answer-
Typically, heat inactivation is used to deactivate serum complement. This product has not been heat inactivated. If this product is to be used in applications where serum complement may interfere, such as antibody-mediated complement cytotoxicity, heat inactivation is often preferred. If the serum complement is not an issue in any downstream assays, heat inactivation is not necessary. This would be up to the end user to determine.
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