The GST tag is located at the N terminus of the protein. unable to share whether this protein contains a linker or not as this is considered proprietary.
S0439
SUMO-1 human
≥95% (SDS-PAGE), recombinant, expressed in E. coli (GST-tagged)
Synonym(s):
Sentrin-1
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| Size/SKU | Availability | Price |
|---|---|---|
500 μg | Please contact Customer Service for Availability | CZK 10,400.00 |
About This Item
CZK 10,400.00
Please contact Customer Service for Availability
recombinant
expressed in E. coli (GST-tagged)
Quality Segment
assay
≥95% (SDS-PAGE)
mol wt
38.5 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SUMO1(7341)
General description
Small ubiquitin-like modifier 1 (SUMO-1), a protein, is one of the 5 SUMO paralogs expressed in mammalian cells with a low but significant homology with ubiquitin.[1] This SUMO-1-GST fusion protein is produced from a DNA sequence corresponding to human SUMO-1 fused to a glutathione S-transferase (GST)-tag and is expressed in E. coli cells.
Application
SUMO-1 human has been used in pre-absorption for negative immunostaining to confirm the specificity of the small ubiquitin like modifier 1 (SUMO-1) antibody.[2]
Biochem/physiol Actions
Conjugated to a variety of proteins by UbcH9, including RanGAP1, IκBα, and PML without the requirement of an equivalent E3 ubiquitin ligase.
Small ubiquitin-like modifier 1 (SUMO-1) plays a key role in modulating survival, migration, and signal transduction in some cell lines. It can prevent in vitro migration and invasion of RA fibroblast-like synoviocytes (FLSs). Higher expression of SUMO-1 may result in joint destruction in rheumatoid arthritis (RA).[3]
Physical form
Solution in 50 mM HEPES, pH 8.0, 150 mM NaCl and 1 mM DTT.
1 of 1
This Item | |||
|---|---|---|---|
| recombinant expressed in E. coli (GST-tagged) | recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli |
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay - | assay ≥90% (SDS-PAGE) |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| shipped in dry ice | shipped in ambient | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 38.5 kDa | mol wt 27 kDa | mol wt antigen ~38.6 kDa | mol wt 27 kDa |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Is S0439-1MG (SUMO-1 human) N- or C-terminal GST tagged? Is there any form of linker amino acids between the SUMO-1 sequence and the GST sequence?
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