Proteins cannot be transferred to a membrane after being stained and de-stained with the Product RPROB, Reversible Protein Detection Kit.
RPROB
Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels
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1 KIT
CZK 7,410.00
CZK 7,410.00
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Technique(s):
protein staining: suitable
Usage:
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
Storage temp.:
2-8°C
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usage
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
technique(s)
protein staining: suitable
storage temp.
2-8°C
Quality Level
Application
R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.
Other Notes
Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.
Kit Components Only
Product No.
Description
- Solution A 500 mL
- Solution B 500 mL
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
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Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)
Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Protocols
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
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Instructions
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When using Product RPROB, Reversible Protein Detection Kit, to stain protein on PAGE gels, can the protein be transferred from the gel to a membrane after destaining?
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