RAB0397

Human PDGF-BB ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(s): PDGF subunit B, PDGF-2, Platelet-derived growth factor subunit B
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... PDGFB(5155)
• Input: sample type serum; sample type plasma; sample type cell culture supernatant(s); sample type urine
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type serum; sample type plasma; sample type cell culture supernatant(s); sample type urine
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 1 pg/mL; standard curve range: 0.549-400 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PDGFB(5155)

Description

General description

The Human PDGF-BB ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF-BB in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PDGF-BB

Application

For research use only. Not for use in diagnostic procedures.
Human PDGF-BB ELISA Kit has been used to measure PDGF-BB (platelet-derived growth factor BB) concentration in human coronary artery smooth muscle cells (HCASMCs).[1]

Biochem/physiol Actions

PDGF-BB (platelet-derived growth factor BB) stimulates lymphatic metastasis and intratumoral lymphangiogenesis.[2] PDGF-BB might be implicated in regulation of angiogenesis and endothelial proliferation.[3] PDGF-BB expressed in placental endothelial cell activates signal transducer and activator of transcription 3 (STAT3) pathway and recruits human placental multipotent mesenchymal stromal cells (hPMSCs) for placental vascularization.[4] Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.[5]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids