RAB0309

Mouse IL-6 ELISA Kit

for cell and tissue lysates

• Synonym(s): B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: mouse ... Il6(24498)
• Input: sample type tissue lysate; sample type cell lysate
• Species reactivity: mouse
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type tissue lysate; sample type cell lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 2 pg/mL; standard curve range: 0.82-600 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Il6(24498)

Description

General description

The Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-6 cell lysate and tissue lysate.

IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3.

Immunogen

Recombinant Mouse IL-6

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse IL-6 ELISA Kit has been used to measure the concentration of interleukin-6 (IL-6) in cell culture supernatants.

Biochem/physiol Actions

IL-6 (interleukin 6) is a pleiotropic cytokine that mediates immune and inflammatory responses. It synergizes with IL-3 in megakaryocyte development and platelet production. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease and plasmacytoma/multiple myeloma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABDIL6

  ELISA 5X Sample Diluent Buffer (Item D2)

  SDS
• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDS
• RABLYSIS1

  2X Cell Lysis Buffer (Item J)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids