RAB0300

Mouse IL-4 ELISA Kit

for cell and tissue lysates

• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 1 pg/mL; standard curve range: 0.819-200 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Il4(16189)

Description

General description

The Mouse IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-4 in cell lysate and tissue lysate.

Immunogen

Recombinant Mouse IL-4

Application

For research use only. Not for use in diagnostic procedures.
Mouse IL-4 ELISA kit has been used for determining the levels of IL-4 in serum sample.

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

IL-4 (interleukin 4) is a pleiotropic cytokine. It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4⁺ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABDIL6 ELISA 5X Sample Diluent Buffer (Item D2)
• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABLYSIS1 2X Cell Lysis Buffer (Item J)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable