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RAB0082

Human CD163 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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1 kit
Please contact Customer Service for Availability
14 000,00 CZK

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... CD163(9332)
Input:
sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

14 000,00 CZK


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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 32.77-8000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CD163(9332)

General description

The Human CD163 (also known as hemoglobin scavenger receptor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD163 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CD163

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human CD163 ELISA kit has been used to measure the levels of CD163 in plasma samples and cell culture supernatants.

Biochem/physiol Actions

Cluster of differentiation 163 (CD163) is a scavenger receptor. It is expressed on the surface of activated macrophages. This protein modulates anti-inflammatory functions and has a role in hemoglobin-haptoglobin clearance. Various cytokines influence the expression of CD163. It may be associated with hepatocellular carcinoma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0081RAB0062RAB0430
Gene Information

human ... CD163(9332)

Gene Information

human ... CD14(929)

Gene Information

human ... CCL21(6366)

Gene Information

human ... SHH(6469)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

input

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

input

sample type cell culture supernatant(s)
sample type urine
sample type plasma
sample type serum

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

input

sample type cell culture supernatant(s)
sample type serum
sample type urine
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 32.77-8000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 32.76-8000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 42 pg/mL
standard curve range: 40.96-10000 pg/mL
(Assay Diluent C), inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 40.96-10000 pg/mL
(Assay Diluent B)

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids



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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  
  1. Does the CD163 ELISA test for the soluble domain?

    1 answer
    1. Epitope mapping has not been conducted. The capture and detection antibodies are Monoclonal Mouse IgG1. The immunogen used is mouse myeloma cell line NS0-derived recombinant human CD163 (aa41-1045), with the accession number CAA80543.

      Helpful?

  2. Is it possible to analyze plasma containing EDTA and cerebrospinal fluid (CSF) using the RAB0082?

    1 answer
    1. According to the supplier, Ray Biotech, plasma samples containing EDTA are not suitable for use in this assay due to its chelating property, as noted in the kit manual. Additionally, the assay has not been directly tested with cerebrospinal fluid (CSF), so its compatibility with CSF cannot be confirmed.

      Helpful?

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