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RAB0037

Human Procalcitonin ELISA Kit

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1 kit
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14 200,00 CZK

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... CALCA(796)
Input:
sample type cell culture supernatant(s)
sample type urine
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

14 200,00 CZK


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description

for cell culture supernatant and urine. Note :The detection range of this kit is 30 pg/ml - 20000 pg/ml.  Procalcitonin is low in normal serum and may be undetectable with this assay.

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CALCA(796)

General description

The Human Procalcitonin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Procalcitonin in cell culture supernatants and urine. Note: The detection range of this kit is 30 pg/ml - 20000 pg/ml.  Procalcitonin is low in normal serum and may be undetectable with this assay.

Immunogen

Recombinant Human Procalcitonin

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Procalcitonin or calcitonin related polypeptide a is a 116-amino acid precursor of calcitonin. It is secreted by monocytes activated by endotoxins or pathogens. The protein is a biomarker for sepsis and bacterial infections. Procalcitonin has been associated with hepatocyte dysfunction.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0632RAB0648RAB0636
Gene Information

human ... CALCA(796)

Gene Information

human ... CALB1(793)

Gene Information

human ... FGA(2243)

Gene Information

human ... CD79A(973)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL

assay range

inter-assay cv: <10%
intra-assay cv: <12%

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.08 pg/mL
standard curve range: 0.082-60 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.65 ng/mL
standard curve range: 0.614-150 ng/mL

input

sample type cell culture supernatant(s)
sample type urine

input

-

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer D (Item K)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids



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Questions

  1. Can the procalcitonin kit used for Human Sputum

    1 answer
    1. This product has not been validated for sputum. For sputum samples, it is recommended to add a protease inhibitor cocktail at a concentration of 1:500. Centrifugation should be performed at 10,000 rpm for 10 minutes, and the supernatant should be diluted 1:2 with sample diluent buffer D prior to assay setup. If necessary, samples can be ultra-centrifuged for 4 hours at 38,000 rpm.

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