R7876
Ribonucleic acid, transfer from wheat germ
Type V, 15-19 units/mg solid
Synonym(s):
Transfer RNA, tRNA
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| Size/SKU | Availability | Price |
|---|---|---|
500 units | Please contact Customer Service for Availability | CZK 4,890.00 |
2500 units | Please contact Customer Service for Availability | CZK 14,200.00 |
10000 units | Please contact Customer Service for Availability | CZK 21,100.00 |
About This Item
Form:
lyophilized powder
Storage condition:
desiccated
CZK 4,890.00
Please contact Customer Service for Availability
grade
Molecular Biology
Quality Segment
type
Type V
form
lyophilized powder
specific activity
15-19 units/mg solid
storage condition
desiccated
solubility
H2O: ~2 mg/mL, clear to hazy
storage temp.
2-8°C
General description
Wheat germ tRNA contains many different specific tRNA molecules.
Application
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.
Preparation Note
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Other Notes
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
1 of 1
This Item | |||
|---|---|---|---|
| form lyophilized powder | form lyophilized powder | form buffered aqueous solution | form lyophilized powder |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: ~2 mg/mL, clear to hazy | solubility - | solubility - | solubility water: 10 mg/mL, clear to slightly hazy, colorless to light yellow |
| storage condition desiccated | storage condition - | storage condition - | storage condition - |
| grade Molecular Biology | grade - | grade Molecular Biology | grade - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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