R5258
Riboflavin Binding Protein from chicken egg white
lyophilized powder
Synonym(s):
Binding Protein, Chicken Egg White Protein, Riboflavin Protein
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2 MG
CZK 1,750.00
10 MG
CZK 4,900.00
CZK 1,750.00
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2 MG
CZK 1,750.00
10 MG
CZK 4,900.00
About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
CZK 1,750.00
Please contact Customer Service for Availability
Recommended Products
biological source
chicken egg white
Quality Level
form
lyophilized powder
concentration
≥50% protein (biuret)
UniProt accession no.
storage temp.
−20°C
Gene Information
chicken ... RBP(396449)
General description
Riboflavin Binding Protein (RBP) comprises of 19 cysteines and corresponds to a molecular weight of 30 kDa.[1] Egg white RBP is produced in the oviduct cells. Chicken egg white RBP contains an N-terminal ligand binding domain and a C-terminal phosphorylated motif.[2]
Riboflavin binding protein (RBP) transports riboflavin through the bloodstream of the laying hen into the eggs, where the vitamin is essential for embryonic growth and development. RBP consists of a single polypeptide chain containing 9 disulfide bonds. It is a glycoprotein containing ~14% carbohydrate, with sugar moieties including galactose, mannose and glucosamine, and is phosphorylated.
Application
Riboflavin Binding Protein from chicken egg white may be used in fluorescence quenching experiments.[1]
Biochem/physiol Actions
Riboflavin Binding Protein (RBP) is crucial for normal embryo development.[1] RBP interacts with riboflavin and its analogs. The RBP-riboflavin complex is stabilized by interaction between the isoalloxazyne ring of riboflavin and the tyrosine 75 and tryptophan 156 of residues of RBP.[3] RBP elicits sweet supressing functionality and may interact with the sweet taste receptor.[4]
Riboflavin binding protein binds to flavokinase and regulates its activity. [5]
Preparation Note
Supplied as the riboflavin-depleted apo-form.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Electrochemistry of riboflavin-binding protein and its interaction with riboflavin
Bartovsik M, et al.
Bioelectrochemistry, 76(1-2), 70-75 (2009)
The effect of riboflavin binding protein (RPB) on flavokinase catalytic activity.
Slomczynska, M.
Comparative Biochemistry and Physiology, 87, 681-685 (1987)
Riboflavin-binding protein exhibits selective sweet suppression toward protein sweeteners
Maehashi K, et al.
Chemical Senses, 32(2), 183-190 (2006)
Binding study of riboflavin-binding protein with riboflavin and its analogues by differential scanning calorimetry
Wasylewski M
Journal of Protein Chemistry, 19(6), 523-528 (2000)
Crystal structure of chicken riboflavin-binding protein
Monaco HL
The Embo Journal, 16(7), 1475-1483 (1997)
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