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R4130

RPMI-1640 Medium

HEPES Modification, with L-glutamine and 25mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

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Size/SKUAvailabilityPrice
10 L
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CZK 2,740.00
10 x 1 L
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CZK 2,800.00
50 L
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CZK 12,500.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.75
MDL number:

CZK 2,740.00


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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Preparation Note

Formulated to contain 16.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.

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This Item
R7513R1145R1780
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

liquid

form

liquid

form

liquid

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

components

HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

400

Quality Level

400

Quality Level

400


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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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