No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.
PIC0006
Protease Inhibitor Cocktail
ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, Non-freezing solution
Synonym(s):
Protease inhibitor cocktail
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Please contact Customer Service for Availability | 4 540,00 CZK |
5 mL | Please contact Customer Service for Availability | 14 700,00 CZK |
About This Item
UNSPSC Code:
12352200
NACRES:
NA.28
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
4 540,00 CZK
Please contact Customer Service for Availability
Product Name
ReadyShield® Protease Inhibitor Cocktail, for use in tissue culture media, Non-freezing solution
description
Tissue Culture Media
Quality Segment
form
ready-to-use solution
quality
200
packaging
pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
input
mammalian sample(s)
solubility
water: soluble
storage temp.
−20°C
General description
This product is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0006 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P1860.
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- Inhibits serine, cysteine, aspartic proteases and aminopeptidases.
- Tailored for use in tissue culture media.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
It is recommended to determine the appropriate dilution for each specific cell line. This testing should begin with a dilution of at least a 200-fold to prevent deleterious effect of the solvent to cell growth.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| description for use in tissue culture media, Non-freezing solution | description Safe for Proteomic Mass Spectrometry applications | description For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase | description for use with mammalian cell and tissue extracts, DMSO solution |
| form ready-to-use solution | form ready-to-use solution | form ready-to-use solution | form DMSO solution |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging - | packaging pkg of 5 mL (in amber polypropylene vial), pkg of 1 mL (in amber polypropylene vial) | packaging - |
| input mammalian sample(s) | input general use sample(s) | input mammalian sample(s) | input mammalian sample(s) |
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Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Do ReadyShield® inhibitor cocktails include EDTA?
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What is the recommended storage temperature for ReadyShield® products?
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The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).
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