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PIC0005

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, For plant cell and tissue extracts, Non-freezing solution

Synonym(s):

Protease inhibitor cocktail

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Size/SKUAvailabilityPrice
1 mL
Please contact Customer Service for Availability
CZK 2,690.00
5 mL
Please contact Customer Service for Availability
CZK 9,190.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C

CZK 2,690.00


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Product Name

ReadyShield® Protease Inhibitor Cocktail, For plant cell and tissue extracts, Non-freezing solution

Quality Segment

description

Plant Cell and Tissue Extracts

form

ready-to-use solution

quality

200

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

solubility

water: soluble

storage temp.

−20°C

General description

This protease inhibitor cocktail has been optimized and tested for plant cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from various plant tissues. Whole extracts of plant seedlings from pea (Pisum sativum), bean (Phaseolus vulgaris), wheat (Triticum aestivum), tobacco (Nicotiana tobaccum), and arabidopsis (Arabidopsis thaliana) have been tested. Extracts of leaves or roots from pea, wheat, and tobacco have also been tested.This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0005 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P9599

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Tailored for Plant cell and tissue extracts.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 30 g (wet weight) of various plant tissues.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
PPC2020PIC0007P9599
form

ready-to-use solution

form

ready-to-use solution

form

ready-to-use solution

form

DMSO solution

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

packaging

-

packaging

-

quality

200

quality

-

quality

-

quality

-

description

Plant Cell and Tissue Extracts

description

-

description

-

description

-


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Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. Hello. Can I store it at -80C? thank you

    1 answer
    1. The stability of this product at -80 °C has not been determined. This product is shipped on wet ice, and storage at -20 °C is recommended. The product is assigned a retest period of 2 years from the date of quality release. Refrigeration at 2-8 °C is acceptable for short term storage. Please see the link below to review additional information available in the product datasheet:https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/256/pic0005-pis-ms.pdf

      Helpful?

  2. Do ReadyShield® inhibitor cocktails include EDTA?

    1 answer
    1. No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.

      Helpful?

  3. What is the recommended storage temperature for ReadyShield® products?

    1 answer
    1. The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).

      Helpful?

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