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P9743

pT7-FLAG™-4 Expression Vector

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10 μg
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9 500,00 CZK

About This Item

NACRES:
NA.32
UNSPSC Code:
41106610

9 500,00 CZK

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shipped in

dry ice

Quality Segment

storage temp.

−20°C

Application

pT7-FLAGa-4 is a 6451 bp Escherichia coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAGÒ fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORF-FLAG fusion constructs.

Legal Information

pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC

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description

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description

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description

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shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ2178

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Questions

  1. pT7-FLAG-4のVectorに遺伝子を導入して導入遺伝子-FLAGごと別のVectorに移動することを考えているのですが、FLAGよりもC-Terminal側の制限酵素サイトの情報はありますでしょうか。ご教示いただけると幸いです。

    1 answer

    1. Only restriction enzyme information at the multiple cloning site (MCS) is available, as shown in the technical bulletin. There isn't a restriction enzyme site available at the C-terminal end of the FLAG tag before the stop codon.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/236/048/p9743bul.pdf

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