For use in Equine sources, Protein G has a significantly higher affinity for IgG, as compared to protein A. Please see the link below to review a comparison table of Protein A to Protein G by species.
P8651
Protein A–Peroxidase from Staphylococcus aureus/horseradish
lyophilized powder
Synonym(s):
Peroxidase–Protein A
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| Size/SKU | Availability | Price |
|---|---|---|
0.5 mg | Please contact Customer Service for Availability | CZK 5,250.00 |
1 mg | Please contact Customer Service for Availability | CZK 8,880.00 |
2 mg | Please contact Customer Service for Availability | CZK 11,400.00 |
About This Item
Form:
lyophilized powder
General description
Protein A is a cell wall constituent of Staphylococcus aureus, with a molecular weight of 56kDa. It possesses five binding domains which interact with heavy chains of immunoglobulins at the Fc or Fab fragment. In response to protein A, the complement system, interferon production, hypersensitivity reactions and cell-mediated cytotoxicity get activated. Horseradish peroxidase is a heme-containing enzyme. In the presence of hydrogen peroxide, the enzyme catalyzes the oxidation of luminol.
Application
Protein A–Peroxidase from Staphylococcus aureus/horseradish has been used in enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Binds IgG only from most mammals, except rat, goat, sheep.
Packaging
Package size based on protein content.
Physical form
Lyophilized powder containing sodium citrate buffer salt. Protein determined by A205.
Preparation Note
Protein A, extracellular, coupled to Peroxidase, Type VI, by a modification of the procedure of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978), which favors low molecular weight conjugates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| technique(s) direct ELISA: 1:80,000 using human IgG | technique(s) direct ELISA: 1:100,000 using human IgG | technique(s) - | technique(s) - |
| form lyophilized powder | form lyophilized powder | form cell suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate unconjugated | conjugate biotin conjugate |
| Quality Level 100, 200 | Quality Level 200 | Quality Level 200 | Quality Level 200, 300 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hello, I would like to know if this conjugate serve for testing equine in a ELISA (Equus ferus caballus). Thank you
1 answer-
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How do I prepare a stock solution of Product P8651, Protein A Peroxidase?
1 answer-
A stock solution of this product can be prepared in PBS (phosphate buffered saline) or other neutral buffer at 1 mg/mL.
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How do I use Product P8651, Protein A Peroxidase, as a my secondary in Western blot analysis?
1 answer-
For Western blotting, with chemiluminescent substrate, dilute 1:1000 to 1:10,000.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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I bought Product P8651, Protein A Peroxidase, to hide heavy and light chain in a Western blot. How does it do that?
1 answer-
P8651 will only recognize the native antibody incubated on a blot, not what's run in the gel. This should circumvent heavy and light chain detection.
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What is the solution stability of Product P8651, Protein A Peroxidase?
1 answer-
The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20 °C.
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