P8412

o-Phenylenediamine dihydrochloride

chromogenic, tablet

• Synonym(s): 1,2-Phenylenediamine tablet, OPD, OPD Tablet
• Linear Formula: C₆H₄(NH₂)₂·2HCl
• CAS Number: 615-28-1
• Molecular Weight: 181.06
• NACRES: NA.83
• PubChem Substance ID: 24898919
• UNSPSC Code: 12352204
• MDL number: MFCD00012966
• Beilstein/REAXYS Number: 3912045

Properties

• Product Name: o-Phenylenediamine dihydrochloride, tablet, 30 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• technique(s): protein detection: suitable
• mp: 258 °C (dec.) (lit.)
• solubility: water: 1 tablet/10 mL, clear, colorless
• storage temp.: 2-8°C
• SMILES string: Cl[H].Cl[H].Nc1ccccc1N
• InChI: 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• InChI key: RIIWUGSYXOBDMC-UHFFFAOYSA-N

Description

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

o-Phenylenediamine dihydrochloride is a binucleophile and its condensation with α,β-unsaturated carbonyl compounds and aldehydes yields benzimidazoles.

Application

o-Phenylenediamine dihydrochloride has been used as a substrate for horse radish peroxidase in detecting:

• human immunoglobulin
• CTLA4Ig in serum samples
• albumin in culture medium

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Safety Information

• Pictograms: GHS08,GHS07,GHS09
• Signal Word: Warning
• Hazard Codes: H302 + H332,H317,H319,H341,H351,H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3