The activity of this product can be measured using a colorimetric assay that quantifies the liberation of choline from L-?-phosphatidylcholine as the substrate. The reaction buffer used is a sodium acetate buffer solution, pH 5.6 at 30°C. Detail of this assay protocol is available on the following web site: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/320/272/p4912enz.pdf?srsltid=AfmBOopflybZ1u1LK4iLu-Wk3wGh2tCFmYL9TfZ8H45Ww4Mwb97rBZ08
P8398
Phospholipase D from cabbage
Type IV, lyophilized powder, ≥100 units/mg solid
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage
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| Size/SKU | Availability | Price |
|---|---|---|
500 units | Please contact Customer Service for Availability | 5 760,00 CZK |
2500 units | Please contact Customer Service for Availability | 21 400,00 CZK |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
EC Number:
Specific activity:
≥100 units/mg solid
5 760,00 CZK
Please contact Customer Service for Availability
type
Type IV
Quality Segment
form
lyophilized powder
specific activity
≥100 units/mg solid
composition
Protein, ~25%
storage temp.
−20°C
General description
Application
Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies.
Biochem/physiol Actions
The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Analysis Note
Protein determined using biuret.
Other Notes
One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
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This Item | |||
|---|---|---|---|
| description Type IV, lyophilized powder, ≥100 units/mg solid | description Type VII, lyophilized powder, ≥150 units/mg solid | description ≥50,000 units/mL, buffered aqueous glycerol solution | description Type II, lyophilized powder, ≥60 units/mg protein |
| specific activity ≥100 units/mg solid | specific activity ≥150 units/mg solid | specific activity ≥50,000 units/mL | specific activity ≥60 units/mg protein |
| form lyophilized powder | form lyophilized powder | form buffered aqueous glycerol solution | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| type Type IV | type Type VII | type - | type Type II |
| composition Protein, ~25% | composition - | composition - | composition Protein, ~30% |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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How to measure the activity of PLD? Is there any specific buffer needed here? In my experiment, phosphatidylcholine cannot be hydrolyzed. Please give me a protocal to measure the activity of PLD.
1 answer-
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How long will it remain stable after reconstitution at 4 degrees Celsius?
1 answer-
Upon the addition of crystalline serum albumin (10 mg/mL) and storage at -15°C, solutions of this product in water are stable for several months. Additional information can be found in the Methods in Enzymology XIV, 202 (1969).
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