P8279
Protocatechuate 3,4-Dioxygenase from Pseudomonas sp.
lyophilized powder, ≥3 units/mg solid
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| Size/SKU | Availability | Price |
|---|---|---|
25 units | Please contact Customer Service for Availability | CZK 17,000.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥3 units/mg solid
Biological source:
bacterial (Pseudomonas spp.)
biological source
bacterial (Pseudomonas spp.)
Quality Segment
form
lyophilized powder
specific activity
≥3 units/mg solid
mol wt
~700 kDa
shipped in
dry ice
storage temp.
−20°C
General description
Structure : Protein with nonheme iron
Inhibitors : Ag+, Hg++, PCMB
Optimum pH : 9.0
Optimum temperature : 60−65°C
pH Stability : pH 7.0−9.0 (25°C, 72hr)
Thermal stability : below 50°C (pH 6.0, 1hr)
Inhibitors : Ag+, Hg++, PCMB
Optimum pH : 9.0
Optimum temperature : 60−65°C
pH Stability : pH 7.0−9.0 (25°C, 72hr)
Thermal stability : below 50°C (pH 6.0, 1hr)
Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe3+.
Application
Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments[1] and is used to study the metabolism of protocatechuate in Rhizobiaceae[2].
The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes.[3] It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.[4]
Biochem/physiol Actions
Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate.
Physical form
Supplied as lyophilized powder.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will oxidize 1.0 μmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥3 units/mg solid | specific activity ≥5 U/mg | specific activity ≥2 units/mg protein | specific activity ≥200,000 units/g protein |
| biological source bacterial (Pseudomonas spp.) | biological source - | biological source - | biological source - |
| form lyophilized powder | form liquid | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~700 kDa | mol wt ~41.8 kDa | mol wt - | mol wt ~300 kDa |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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