P8047

Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−R-Phycoerythrin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Goat Anti-Human IgG
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162448
• Conjugate: phycoerythrin (R-PE) conjugate
• Clone: polyclonal
• Application: IF (d)
• Citations: 13

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: phycoerythrin (R-PE) conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct immunofluorescence: 1:32
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment R-Phycoerythrin antibody produced in goat has been used in bead-based assay and immunoassay multiplex magpix

Anti-Human IgG (γ-chain specific), F(ab′)² fragment-R-Phycoerythrin antibody is suitable for use in flow cytometry . The antibody can also be used for direct immunofluorescence (1:32).

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises the H chain constant region.

IgG antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The use of anti-human IgG (γ-chain specific), F(ab′)² fragment-R-Phycoerythrin antibody helps avoid background staining due to the presence of Fc receptors. The product is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ, and Bence Jones λ myeloma proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.1 mM EDTA, 1 mM iodoacetamide, 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)