Dissolve this product in cold 50 mM Tris-maleate buffer containing 1% bovine serum albumin at pH 7.3. Prepare the solution immediately before use. Long-term storage of the reconstituted solution at -80 °C has not been validated. For long-term storage, freeze the solution promptly in single-use aliquots, avoid repeated freeze-thaw cycles, and confirm enzyme activity after thawing. Store the unopened lyophilized product at -20 °C.
P7633
Phospholipase C from Clostridium perfringens (C. welchii)
Type I, lyophilized powder, 10-50 units/mg protein
Greener Alternative
Synonym(s):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
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| Size/SKU | Availability | Price |
|---|---|---|
25 units | Please contact Customer Service for Availability | 2 870,00 CZK |
125 units | Please contact Customer Service for Availability | 3 880,00 CZK |
500 units | Please contact Customer Service for Availability | 11 400,00 CZK |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-638-2
MDL number:
Specific activity:
10-50 units/mg protein
Biological source:
bacterial (Clostridium perfringens)
2 870,00 CZK
Please contact Customer Service for Availability
biological source
bacterial (Clostridium perfringens)
Quality Segment
type
Type I
form
lyophilized powder
specific activity
10-50 units/mg protein
composition
protein, 20-40%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
application(s)
diagnostic assay manufacturing
greener alternative category
, Enabling
storage temp.
−20°C
Gene Information
Clostridium perfringens str. 13 ... plc(988262)
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Biochem/physiol Actions
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol. This enzyme also hydrolyzes the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid.
Other Notes
One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| description Type I, lyophilized powder, 10-50 units/mg protein | description powder, white, ≥200 U/mg | description lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose | description 5 mg protein/mL |
| biological source bacterial (Clostridium perfringens) | biological source bacterial (Pseudomonas spp.) | biological source - | biological source bovine heart |
| Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information - | Gene Information - | Gene Information - |
| specific activity 10-50 units/mg protein | specific activity ≥200 U/mg | specific activity 30-60 units/mg protein (biuret) | specific activity ≥20 units/mg protein |
| form lyophilized powder | form powder | form lyophilized powder | form buffered aqueous solution |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Could you please advise on the recommended buffer for the initial dissolution of P7633? Also, if I prepare a stock solution, is it appropriate to store it directly at -80°C for long-term storage? I would appreciate it if you could provide the recommended
1 answer-
Helpful?
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what is the meaning of 10-50 units/mg protein.
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The protein content and enzymatic activity of this product vary from lot to lot. The protein content will range from 20% - 40%, and the activity will range from 10 units - 50 units per milligram of protein. As an example, a batch that reports a protein content of 20% and an activity of 10 units per milligram of protein will have a vial content of 12.5 mg for the 25 unit package size. The protein content and activity are reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/p7633#product-documentationHelpful?
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P7633とP4039の精製方法の概要（方法名のみで差し支えございません）を開示して頂くことは可能でしょうか？
1 answer-
Unfortunately, the purification method for these products are considered proprietary. However, the following reference may be of interest:
Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein. T Takahashi et al. Biochimica et biophysica acta, 351(1), 155-171 (1974-05-10)
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What are the differences in the purification methods between Products P7633 and P4039 Phospholipase C from Clostridium perfringens (C. welchii)?
1 answer-
The extraction and purification methods for our Phospholipase C products are proprietary, however higher unit per milligram values indicate a more purified product. Product P7633 contains a specific activity of 10-50 units/mg protein and the protein content is between 20.0 - 40.0 %. Product P4039 will contain a specific activity of ≥150 units/mg protein. The protein content for this product is between 5.0 - 30.0 %. The impact of the methods on the isoform profile of these products is not evaluated. The specific activity of each lot activity will be reported in the Certificate of Analysis. Please view the sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P7633-500UN0000218762.pdf
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4039-125UN0000185061.pdfHelpful?
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Are Products P7633 and P4039, Phospholipase C from Clostridium perfringens (C. welchii), prepared from the same strain?
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Products P7633 and P4039, Phospholipase C, from Clostridium perfringens (C. welchii) are prepared from the same strain.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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