The enzyme can be solubilized in 10 mM Tris-HCl, pH 7.4, containing 144 mM NaCl and 0.05% BSA. Store this product at –20 °C. The lyophilized product, when stored properly, retains activity for at least 2 years. A preparation of this enzyme in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0, and 10 mM EDTA retains activity at 2–8 °C for at least 3 years. Please see the link below to review additional product information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/218/p5542dat.pdf
P5542
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
lyophilized powder, ≥1,000 units/mg protein (Lowry)
Synonym(s):
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
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| Size/SKU | Availability | Price |
|---|---|---|
5 units | Please contact Customer Service for Availability | CZK 6,940.00 |
25 units | Please contact Customer Service for Availability | CZK 23,800.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
form
lyophilized powder
Quality Segment
specific activity
≥1,000 units/mg protein (Lowry)
foreign activity
Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein
storage temp.
−20°C
Gene Information
Bacillus cereus E33L ... BCZK3513(3026815)
General description
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.
Application
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane.[1] It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.
Biochem/physiol Actions
Used for the release of GPI anchored proteins from the membrane.
Physical form
Contains phosphate buffer salts, EDTA and stabilizer
Analysis Note
Acetylcholinesterase is measured according to Ellman, et al.
Other Notes
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
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This Item | |||
|---|---|---|---|
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| form lyophilized powder | form buffered aqueous glycerol solution | form solid | form lyophilized powder |
| Quality Level 300 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| Gene Information Bacillus cereus E33L ... BCZK3513(3026815) | Gene Information Bacillus cereus E33L ... BCZK3513(3026815) | Gene Information - | Gene Information Bacillus cereus ATCC 10987 ... plC(2749087) |
| foreign activity Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein | foreign activity Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein | foreign activity - | foreign activity - |
| specific activity ≥1,000 units/mg protein (Lowry) | specific activity ≥1,000 units/mg protein (Lowry) | specific activity ≥50 units/mg dry wt. | specific activity ≥200 units/mg protein |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Which buffer should I use to reconstitute the enzyme in aqueous form and store at 4C or -20C?
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