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P4109

β-Phosphoglucomutase from Lactococcus sp.

recombinant, expressed in E. coli

Synonym(s):

β-D-Glucose 1,6-phosphomutase

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Size/SKUAvailabilityPrice
250 units
Please contact Customer Service for Availability
CZK 3,580.00
1000 units
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CZK 10,200.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥10 unit/mg solid
Recombinant:
expressed in E. coli

CZK 3,580.00


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recombinant

expressed in E. coli

Quality Segment

form

solid

specific activity

≥10 unit/mg solid

storage temp.

−20°C

Application

β-Phosphoglucomutase is used to study starch and sucrose metabolism. It is used for the determination of α-amylase in clinical analysis. It is used to study galactose utilization[1] and generalized glycogenosis (Pompe′s Disease)[2].

Biochem/physiol Actions

Enzymatically converts β-D-glucose-1-phosphate to β-D-glucose-6-phosphate.
β-Phosphoglucomutase enzymatically converts β-D-glucose 1-phosphate to β-D-glucose 6-phosphate. It is involved in starch and sucrose metabolism.

Other Notes

Contains EDTA and lactose.
One unit is defined as the amount of enzyme which converts 1μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per minute at 37°C and pH 7.0.

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This Item
SRE0069P3397I4504
specific activity

≥10 unit/mg solid

specific activity

≥100 units/mg protein

specific activity

≥100 units/mg protein

specific activity

≥300 units/mg solid

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

solid

form

lyophilized powder

form

ammonium sulfate suspension

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. Hello, I received the enzyme pgmB (P4109) and wondered in what buffer should I dissolve the enzyme powder. Thank you

    1 answer
    1. This product may be reconstituted in 10 mM Potassium Phosphate, pH 7 at 5mg/mL. The stock solution is expected to be stabe for at least 1 week when stored refrigerated. For assay purposes, the enzyme is prepared just prior to use with ice cold 10 mM Potassium Phosphate, pH 7 solution duluted to 1.0 - 3.0 U/mL.

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