Skip to Content
Merck

Key Documents

View All Documentation

Skip To

P4069

Phosphatase, Alkaline from Escherichia coli

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
25 units
Please contact Customer Service for Availability
8 980,00 CZK
100 units
Please contact Customer Service for Availability
26 400,00 CZK

About This Item

CAS Number:
9001-78-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-631-4
MDL number:
MFCD00131849
EC Number:
3.1.3.1 (BRENDA, IUBMB)
eCl@ss:
42010105
Specific activity:
20-50 units/mg protein (in glycine buffer)
Biological source:
Escherichia coli

8 980,00 CZK

Please contact Customer Service for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Escherichia coli

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

20-50 units/mg protein (in glycine buffer)

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Application

Phosphatase, Alkaline from Escherichia coli has been used:
  • in fluorometric assay to determine its enzyme activity
  • in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
  • in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl2, 0.5 mM ZnCl2, 50% (w/v) glycerol

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
P4252P5931A2356
Phosphatase, Alkaline from Escherichia coli buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Sigma-Aldrich

P4069

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from Escherichia coli ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Sigma-Aldrich

P4252

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from Escherichia coli lyophilized powder, 30-60 units/mg protein (in glycine buffer)

Sigma-Aldrich

P5931

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from bovine intestinal mucosa ≥5,500 DEA units/mg protein

Sigma-Aldrich

A2356

Phosphatase, Alkaline from bovine intestinal mucosa

description

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

description

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

description

lyophilized powder, 30-60 units/mg protein (in glycine buffer)

description

≥5,500 DEA units/mg protein

biological source

Escherichia coli

biological source

Escherichia coli

biological source

-

biological source

-

specific activity

20-50 units/mg protein (in glycine buffer)

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

specific activity

30-60 units/mg protein (in glycine buffer)

specific activity

≥5,500 DEA units/mg protein

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

foreign activity

DNase, RNase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

Still not finding the right product?

Explore all of our products under Phosphatase, Alkaline from Escherichia coli

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters