Skip to Content
Merck

P3752

Phosphatase, Acid from potato

lyophilized powder, ≥0.5 unit/mg solid

Synonym(s):

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

Slide 1 of 2
Sign In to View Organizational & Contract Pricing.

Select a Size

100 MG

CZK 1,900.00

1 G

CZK 6,950.00

CZK 1,900.00

Please contact Customer Service for Availability
​

About This Item

CAS Number:
9001-77-8
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
MFCD00131847
EC Number:
3.1.3.2 (BRENDA, IUBMB)
Specific activity:
≥0.5 unit/mg solid
Biological source:
potato

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

SMILES string

O(CC(O)C)CC#C

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

biological source

potato

form

lyophilized powder

specific activity

≥0.5 unit/mg solid

mol wt

69 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P1146SRE0048P1435
Phosphatase, Acid from potato lyophilized powder, ≥0.5 unit/mg solid

Sigma-Aldrich

P3752

Phosphatase, Acid from potato

Phosphatase, Acid from potato lyophilized powder, ≥3.0 units/mg solid

Sigma-Aldrich

P1146

Phosphatase, Acid from potato

Sigma-Aldrich

Sigma-Aldrich

SRE0048

Phosphatase, Acid from potato

Phosphatase, Acid from sweet potato ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)

Sigma-Aldrich

P1435

Phosphatase, Acid from sweet potato

biological source

potato

biological source

-

biological source

-

biological source

-

specific activity

≥0.5 unit/mg solid

specific activity

≥3.0 units/mg solid

specific activity

3.0-10.0 unit/mg solid

specific activity

≥10.0 units/mg protein (modified Warburg-Christian)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

mol wt

69 kDa

mol wt

69 kDa

mol wt

-

mol wt

110 kDa

Application

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages[1].

Biochem/physiol Actions

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate[1].

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000521095
0000521095
0000493566
0000493566
0000479100

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

I N Mbawuike et al.
Infection and immunity, 51(2), 483-489 (1986-02-01)
The kinetics of induction of the bronchoalveolar cell population (i.e., alveolar macrophages [AM], lymphocytes, and polymorphonuclear leukocytes) was studied in mice inoculated intravenously with heat-killed Mycobacterium bovis BCG. Injection of BCG at 100 and 500 micrograms but not at 10
Filipe Natalio et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 23(21), 4973-4980 (2017-03-02)
Boron's unusual properties inspired major advances in chemistry. In nature, the existence and importance of boron has been fairly explored (e.g. bacterial signaling, plant development) but its role as biological catalyst was never reported. Here, we show that boric acid
K Zyła et al.
The British journal of nutrition, 74(1), 3-17 (1995-07-01)
An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC 3.1.3.8), and to quantify the effect of acid phosphatase (EC 3.1.3.2), fungal protease (EC 3.4.23.6) and Aspergillus niger cellulase (EC 3.2.1.4)
Julia R Smith et al.
Physiological genomics, 31(2), 357-363 (2007-08-09)
We have developed a method to determine the degree of phosphorylation of a peptide in a complex mixture without enrichment or operation of the mass spectrometer in negative ion mode. Yeast lysate containing known amounts of synthetic peptides (VPQLEIVPNSAEERLHSMK and
Jia Zhang et al.
Biosensors & bioelectronics, 141, 111442-111442 (2019-06-30)
Phenol contains an exchangeable hydroxyl proton resonant at 4.8 ppm from the resonance frequency of water in the 1H nuclear magnetic resonance (1H NMR) spectrum, enabling itself to be detected at sub-mM concentration by either chemical exchange saturation transfer magnetic resonance
View All Related Papers

Protocols

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service