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| Size/SKU | Availability | Price |
|---|---|---|
0.2 mL | Please contact Customer Service for Availability | CZK 2,360.00 |
About This Item
Form:
buffered aqueous solution
Concentration:
2 mg/mL
sterility
non-sterile
Quality Segment
form
buffered aqueous solution
concentration
2 mg/mL
shipped in
dry ice
storage temp.
−20°C
General description
The activity of proteins depends on phosphorylation of specific amino acid residues. In applications involving identification of activated cellular proteins, it is important to confirm specificity of antibodies used against a particular phosphorylated amino acid. For such applications, inhibitors of phosphorylated antibodies on specific amino acid residues are suitable. Phosphoserine antibody inhibitor may be used for the specific inhibition of the reactivity of anti-phosphoserine antibodies but not that of anti-phosphothreonine- or anti-phosphotyrosine-specific antibodies.
Application
Phosphoserine-BSA may be used for specific inhibition of monoclonal anti-phosphoserine (Sigma Product No. P3430) in immunoblotting and ELISA applications, at a working dilution of 2 μg/mL.
Biochem/physiol Actions
O-phospho-L-serine conjugated to bovine serum albumin. Phosphoserine antibody inhibitor specifically blocks the reactivity of anti-phosphoserine antibody. It does not block the activity of anti-phosphothreonine or anti-phosphotyrosine-specific antibodies. The product is useful in immunoblotting and in ELISA.
Working dilution is at least 2 μg/ml by the specific inhibition of the reactivity of Monoclonal Anti-Phosphoserine (Sigma Product No. P3430), used in immunoblotting of rat cerebral cortex extract, and in ELISA.
Working dilution is at least 2 μg/ml by the specific inhibition of the reactivity of Monoclonal Anti-Phosphoserine (Sigma Product No. P3430), used in immunoblotting of rat cerebral cortex extract, and in ELISA.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form buffered aqueous glycerol solution |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| concentration 2 mg/mL | concentration 2 mg/mL | concentration 5—6 mg/mL protein | concentration 250 μg/mL |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in wet ice |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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