P3338

Prostate Specific Antigen from human semen

buffered aqueous solution

• Synonym(s): Cancer-associated PSA, PSA
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00165544
• Form: buffered aqueous solution
• Assay: ≥95% (SDS-GE)
• Biological source: human semen

Properties

• biological source: human semen
• Quality Segment: 200
• assay: ≥95% (SDS-GE)
• form: buffered aqueous solution
• UniProt accession no.: P07288
• storage temp.: 2-8°C
• Gene Information: human ... KLK3(354)

Description

General description

Prostate specific antigen (PSA) is a 28.43 kDa single-chain glycoprotein containing 237 amino acids, encoded by the kallikrein gene mapped to human chromosome 19. It is specifically expressed by human prostate tissue and is one of the highly abundant proteolytic enzyme expressed in the seminal fluid.

Application

Prostate Specific Antigen from human semen has been used:

• in microarray sandwich assay
• in the preparation of sandwich immunosensor
• as a target protein to test composite organic−inorganic nanoparticle (COIN) and Alexa conjugates in a plate-binding assay

Biochem/physiol Actions

Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa. Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA.

Prostate Specific Antigen (PSA), a glycoprotein of the glandular kallikrein family, is a serine protease with chymotrypsin-like enzymatic activity. Circulating PSA exists in free and protease inhibitor-bound forms. Studying the ratios of free to inhibitor-bound forms may prove valuable in the diagnosis of prostate cancer.

Glycoprotein of the glandular kallikrein family

Packaging

Package size based on protein content

Physical form

Solution in phosphate buffered saline, contains 0.05-0.1% sodium azide with 50% glycerol

Analysis Note

Highly purified

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable