P3296
Protein G Sepharose™, Fast Flow
recombinant, expressed in E. coli, aqueous ethanol suspension
Synonym(s):
Protein G-Agarose, Fast Flow from Streptococcus sp.
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About This Item
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recombinant
expressed in E. coli
form
aqueous ethanol suspension
analyte chemical class(es)
proteins (Immunoglobulins of various mammalian species)
extent of labeling
~2 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4B Fast Flow
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
storage temp.
2-8°C
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General description
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
P3296-5Ml′s updated product number is GE17-0618-01
P3296-5Ml′s updated product number is GE17-0618-01
Application
Protein G-Sepharose™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.
Physical form
Suspension in 20% ethanol
Preparation Note
Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted
Legal Information
Sepharose is a trademark of Cytiva
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
115.0 °F - closed cup
Flash Point(C)
46.1 °C - closed cup
Certificates of Analysis (COA)
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Protocols
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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