P2645

Protein Kinase A Catalytic Subunit from bovine heart

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

• Synonym(s): PKA
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.32
• EC Number: 2.7.11.11 (BRENDA, IUBMB)
• MDL number: MFCD00132114
• Specific activity: ≥9 units/μg protein (cyclic-AMP is not required for this activity)

Properties

• form: lyophilized (white powder to sticky mass to hard pellet)
• Quality Segment: 200
• specific activity: ≥9 units/μg protein (cyclic-AMP is not required for this activity)
• mol wt: 40,862 Da
• storage temp.: −20°C

Description

General description

Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.

Application

Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-

• to study PKA-mediated inhibition of IRK1 (inwardly rectifying K⁺) channels
• in in Vitro PKA pPhosphorylation assay
• in Vitro affinity binding assays
• to study effects of PKA on inspiratory drive currents in functionally active motorneurons

Biochem/physiol Actions

Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.

Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.

Packaging

Package size based on phosphorylating units

Physical form

Lyophilized powder with sucrose and phosphate buffer salts as stabilizer.

Preparation Note

Prepared from protein kinase A (P 5511)

Other Notes

Phosphorylating Activity: One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C, determined by measuring the production of ADP.

Disclaimer

Please note that the pack size has been changed to align with the unit definition, while the number of phosphorylating units remain the same as before.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)