The final buffer that this product is in prior to lyophilization is 0.1 M B-glycerophosphate and 4 mM EDTA.
A stock solution can be prepared in 40 mM glycerophosphate buffer, pH 6.8, containing 1 mM EDTA and 5 mM cysteine. EDTA is a chelating agent of copper and other metal ions, which causes oxidation of the protein. The presence of cysteine is to maintain the critical sulfhydryl group in the protein in the reduced state.
When preparing a stock solution for assaying the activity, a 0.25 unit/ml solution of the protein in 40 mM glycerophosphate buffer, pH 6.8, at 30 °C containing 80 mM cysteine is used.
P1261
Phosphorylase a from rabbit muscle
lyophilized powder, 20-30 units/mg protein
Synonym(s):
1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase
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| Size/SKU | Availability | Price |
|---|---|---|
10 mg | Please contact Customer Service for Availability | CZK 5,630.00 |
25 mg | Please contact Customer Service for Availability | CZK 11,500.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-878-8
MDL number:
Specific activity:
20-30 units/mg protein
Biological source:
rabbit muscle
biological source
rabbit muscle
Quality Segment
form
lyophilized powder
specific activity
20-30 units/mg protein
purified by
2× crystallization
composition
Protein, 30-60% E1%/280
foreign activity
AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%, phosphoglucomutase ≤0.25%, phosphorylase b ≤30%
storage temp.
−20°C
General description
Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).
Application
Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.
Biochem/physiol Actions
Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.
Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls. [3]
Physical form
Lyophilized powder containing β-glycerophosphate and EDTA
Other Notes
One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)
1 of 1
This Item | |||
|---|---|---|---|
| biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle |
| specific activity 20-30 units/mg protein | specific activity ≥200 units/mg protein | specific activity ≥20 units/mg protein | specific activity ≥75 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| purified by 2× crystallization | purified by - | purified by 2× crystallization | purified by - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the buffer required for the enzyme activity?
1 answer-
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What is the solubility of Phosphorylase A, Product P1261?
1 answer-
Sigma tests solubility of Phosphorylase A (P1261) in water at a concentration of 1 mg/ml.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Phosphorylase A, Product P1261?
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The molecular weight of Rabbit Phosphorylase A (tetramer) is 370,000 per Biochemistry 6 (1967) p. 3315 (which was determined by sedimentation and "experiments in a variety of buffers, temperatures, and ionic strength"). The molecular weight by sequence analysis (Proc. Natl. Acad. Sci. USA, 74, 4762-4766) is 97,412.
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Has the amino acid sequence of phosphorylase from rabbit muscle been published?
1 answer-
The sequence has been published. The amino acid composition is:Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf).Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric form. The sequence information of the subunit would be the same for both forms.
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How is Phosphorylase A, Product P1261, prepared?
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To prepare Phosphorylase A (P1261), Sigma uses a method similar to the one described in the J. Biol.Chem., 231, 65 (1958).
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What is the final buffer Phosphorylase A, Product P1261, is in prior to lyophilization?
1 answer-
The final buffer Phosphorylase A (P1261) is in prior to lyophilization is 0.1MB-glycerophosphate and 4 mM EDTA.
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What is the pI of Phosphorylase A, Product P1261?
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The pI of Phosphorylase A (P1261) is 5.6 - 5.8.
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