O3514

Bovine Osteopontin

from bovine milk, powder, suitable for cell culture

• Synonym(s): Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD03100698
• Biological source: bovine milk
• Form: lyophilized powder
• Technique(s): cell culture | mammalian: suitable
• Mol wt: ~60 kDa (reducing conditions)
• Assay: >85% (by SDSPAGE under reducing conditions and visualized by silver stain)

Properties

• Product Name: Osteopontin from bovine milk, lyophilized powder
• biological source: bovine milk
• Quality Segment: 100
• sterility: sterile
• assay: >85% (by SDSPAGE under reducing conditions and visualized by silver stain)
• form: lyophilized powder
• mol wt: ~60 kDa (reducing conditions)
• packaging: pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P31098, P31096
• shipped in: ambient
• storage temp.: -10 to -25°C
• Gene Information: cow ... SPP1(281499)

Description

General description

Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells. It has 261 amino acid residues with a molecular mass of 29,283 Da. Osteopontin is also called as uropontin. Osteopontin from bovine milk is highly glycosylated and phosphorylated. SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 and cow chromosome 6. It has seven exons and six introns.

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.

Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses. It binds to av-containing integrins and stimulate cell adhesion and migration. Osteopontin promotes Th1 type immunity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)