Skip to Content
Merck

Key Documents

View All Documentation

Skip To

N8010

4-Nitrophenyl 4-guanidinobenzoate hydrochloride

protease substrate, ≥95.0% (TLC), powder

Synonym(s):

4-Guanidinobenzoic acid 4-nitrophenylester hydrochloride, NPGB, pNPGB

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
500 mg
Please contact Customer Service for Availability
CZK 9,310.00
1 g
Please contact Customer Service for Availability
CZK 12,900.00
CZK 10,965.00

About This Item

Empirical Formula (Hill Notation):
C14H12N4O4 · HCl
CAS Number:
19135-17-2
Molecular Weight:
336.73
NACRES:
NA.32
PubChem Substance ID:
24897847
UNSPSC Code:
12352204
EC Number:
242-831-3
MDL number:
MFCD00037010
Beilstein/REAXYS Number:
8024554

CZK 9,310.00

Please contact Customer Service for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

4-Nitrophenyl 4-guanidinobenzoate hydrochloride, protease inhibitor and substrate

Quality Segment

200

assay

≥95.0% (TLC)

form

powder

solubility

formic acid: soluble 49.00-51.00 mg/mL

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)Nc1ccc(cc1)C(=O)Oc2ccc(cc2)[N+]([O-])=O

InChI

1S/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H

InChI key

PKSBDZOBYIKNGY-UHFFFAOYSA-N

General description

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a trypsin substrate. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assay.[1]

Application

4-Nitrophenyl 4-guanidinobenzoate hydrochloride has been used:
  • as a substrate for trypsin for active site titration experiments
  • for pre-treating of mosquito eggs in the interplasmid transposition assay [2]
  • as a component of isotonic buffer to moisten filter paper for mosquito embryo collection[3]

Biochem/physiol Actions

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.[3]

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
N162773677N2127
4-Nitrophenyl 4-guanidinobenzoate hydrochloride protease inhibitor and substrate

Sigma-Aldrich

N8010

4-Nitrophenyl 4-guanidinobenzoate hydrochloride

4-Nitrophenyl β-D-glucuronide ≥98% (TLC)

Sigma-Aldrich

N1627

4-Nitrophenyl β-D-glucuronide

4-Nitrophenyl β-D-glucuronide ≥99.0% (TLC)

Sigma-Aldrich

73677

4-Nitrophenyl β-D-glucuronide

4-Nitrophenyl α-D-mannopyranoside α-mannosidase substrate

Sigma-Aldrich

N2127

4-Nitrophenyl α-D-mannopyranoside

assay

≥95.0% (TLC)

assay

≥98% (TLC)

assay

≥99.0% (TLC)

assay

≥98% (TLC)

solubility

formic acid: soluble 49.00-51.00 mg/mL

solubility

H2O: soluble 100 mg/mL, clear

solubility

H2O: 0.1 g/mL, clear, faintly yellow

solubility

DMF: 50 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

form

powder

form

powder

form

powder or crystals

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters