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N6655

Anti-Nerve Growth Factor 2.5S antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

Synonym(s):

Anti-NGF-2.5S

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Size/SKUAvailabilityPrice
0.1 mL
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CZK 4,020.00

About This Item

UNSPSC Code:
51111800
EC Number:
210-123-3
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ODD, WB
Citations:
17

CZK 4,020.00


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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

Ouchterlony double diffusion: suitable, western blot: 1:2,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NGF(4803)
mouse ... Ngf(18049)

General description

Nerve growth factor (NGF) belongs to the family of neurotrophic factors. It is located at 1p13.2 on the human chromosome. There are two molecular forms of NGF, 7S NGF and 2.5S NGF, that have identical neurotrophic effect on neurons.

Immunogen

NGF-2.5S from male mouse submaxillary glands.

Application

Anti-Nerve Growth Factor 2.5S antibody may be used for immunoblotting and dot blot at a minimum antibody working dilution of 1:2000. Antibody dilution of 1:1000 was used for immunoblotting using extracts of nucleus raphe magus tissue of rats. The antibody was used for immunofluorescence of mouse astrocytes and for neutralization assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

May be used in monitoring for NGF (2.5S, β and 7S).
Mutations in this gene leads to hereditary sensory and autonomic neuropathies class five (HSAN-V).

Physical form

Solution in phosphate buffered saline.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
N3279N3908N8773
species reactivity

mouse, human

species reactivity

human, rat, mouse

species reactivity

rat

species reactivity

rat

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

goat

antibody form

fractionated antiserum

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

25623, monoclonal

clone

polyclonal

clone

polyclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

UniProt accession no.

P01138

UniProt accession no.

P01138

UniProt accession no.

P07174

UniProt accession no.

P25427


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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–4 of 4 Questions  
  1. Is this antibody N6655 anti-NGF 2.5S against targeting NGFR?

    1 answer
    1. Anti-Nerve Growth Factor 2.5S, catalog number N6655, is produced in rabbits using immunogen 2.5S nerve growth factor purified from mouse submaxillary glands.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. In western blot application, what size protein is recognized by Product N6655, Anti-Nerve Growth Factor 2.5S antibody produced in rabbit?

    1 answer
    1. Product N6655, Anti-Nerve Growth Factor 2.5S antibody produced in rabbit, will recognize a 30 kD protein on western blot.

      Helpful?

  4. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

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