N1905

Neurotrophin-3 human

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

• Synonym(s): HDNF, NTF3, NT-3
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00212404

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in baculovirus infected Sf21 cells
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 1-40 ng/mL EC₅₀
• packaging: pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P20783
• storage temp.: −20°C
• Gene Information: human ... NTF3(4908)

Description

General description

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family. This protein is made up of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.

Application

Neurotrophin-3 human has been used in the magnetic sorting of oligodendrocyte progenitor cells.

Biochem/physiol Actions

Neurotrophin-3 (13.6 kDa or 27.2 kDa dimer) is a member of the neurotrophin family. It has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. NT-3 is important in development and maintenance of neuronal populations and promotes differentiation of neural crest derived sensory and sympathetic neurons. It is critical for proprioceptive 1a afferent neurons, which relay information from peripheral muscle spindles to motoneurons, sending projections to spinocerebellar neurons. NT-3 is also critical in the superior cervical and nodose ganglia.

Physical form

Lyophilized from a 0.2 m filtered solution in phosphate buffered saline (PBS) containing 50 μg of bovine serum albumin (BSA) per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a TrkB-transfected cell line BaF-TrkB-BD.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)