Nile Blue A is available in various forms or salts, such as sulfate, perchlorate, or chloride. Suggestion is to use the chloride form in a similar manner to the sulfate form and make adjustments based on the staining results.
N0766
Nile Blue A
Dye content, ≥75%, certified by the Biological Stain Commission, powder
Synonym(s):
Basic Blue 12, Nile blue sulfate
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| Size/SKU | Availability | Price |
|---|---|---|
5 g | Please contact Customer Service for Availability | CZK 1,760.00 |
25 g | Please contact Customer Service for Availability | CZK 4,660.00 |
About This Item
Linear Formula:
2C20H20N3O · SO4
CAS Number:
Molecular Weight:
732.85
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
222-832-5
MDL number:
Colour Index Number:
51180
Product Name
Nile Blue A, certified by the BSC
agency
certified by the BSC
Quality Segment
form
powder
composition
Dye content, ≥75%
technique(s)
microbe id | staining: suitable
color
dark green
mp
>300 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78
InChI
1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)
InChI key
QIRDPEPUXNCOLD-UHFFFAOYSA-N
Application
Nile Blue A has been used for the staining of eggs before transferring them to the hosts. It has been used for the staining of cells.
Certified for use in Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue; Lillie and Fuller method for fatty acids/lipids using frozen sections of fatty liver and avocado.
Features and Benefits
Used in the preparation of an amperometric glucose sensor.
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This Item | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) titration: suitable | technique(s) microbe id | staining: suitable |
| color dark green | color - | color - | color - |
| form powder | form powder | form powder | form powder, crystals or chunks |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility H2O: 1 mg/mL | solubility H2O: 1 mg/mL, blue | solubility methanol: 1 mg/mL | solubility - |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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