MSQC10

SILu^™MAb Mouse Stable-Isotope Labeled Monoclonal Antibody

from mouse, recombinant, expressed in CHO cells

• NACRES: NA.12
• UNSPSC Code: 12352202
• Technique(s): protein mass spectrometry: suitable
• Species reactivity: -
• Application: protein mass spectrometry
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in CHO cells
• antibody product type: primary antibodies
• assay: ≥90% (SDS-PAGE)
• packaging: vial of 100 μg
• technique(s): protein mass spectrometry: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

SILu^™MAb Mouse is a recombinant, stable isotope-labeled, mouse monoclonal antibody which incorporates [¹³C₆, ¹⁵N₄]-Arginine and [¹³C₆, ¹⁵N₂]-Lysine. Expressed in CHO cells, SILu^™MAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies.

SILu^™MAb Mouse is an IgG1 antibody with a kappa light chain. Because of overlap with common sequences in the Fc region with candidate murine antibodies, SILu^™MAb Mouse provides universal utility, and thus, eliminates the need to produce candidate-specific internal standards.

Physical form

Each vial of SILu^™MAb Mouse contains the labeled antibody lyophilized from a solution of phosphate buffered saline. Vial content was determined by measuring A₂₈₀ and using an extinction coefficient (E_0.1%) of 1.4.

Preparation Note

Produced utilizing enriched media containing stable isotope labeled amino acids such as [¹³C₆, ¹⁵N₄]-Arginine and [¹³C₆, ¹⁵N₂]-Lysine. Expressed in CHO cells, SILuMAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies

SILu^™MAb Mouse recovery is maximized when 0.1% formic acid is used for reconstitution of the lyophilized product.
Reconstitution with other solvents may reduce recovery. Do not freeze after reconstitution.

• Briefly centrifuge the vial at ~10,000 × g to collect the product at the bottom of the vial.
• Add 500 μL of ultrapure water containing 0.1% formic acid to the vial.
• Mix the contents by gently inverting the vial a minimum of 5 times.
• Allow the vial to stand at room temperature for at least 15 minutes and repeat mixing by inversion.

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable