The strain is Enterococcus faecalis (ATCC 12984). The inactivation method is proprietary. Sterility testing is performed to verify that complete inactivation is achieved.
MBD0011
Inactivated Enterococcus faecalis
Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Please contact Customer Service for Availability | 7 280,00 CZK |
About This Item
NACRES:
NA.51
UNSPSC Code:
41105500
7 280,00 CZK
Please contact Customer Service for Availability
Quality Segment
form
liquid
concentration
>10^8 bacteria/ml
technique(s)
DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Inactivated Enterococcus faecalis can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Enterococcus faecalis is a gram positive, facultative anaerobic, non-motile, coci-shaped bacterium. It is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients. The pathogenesis of E. faecalis infection relies in part on its capacity to colonize the gut. Following disruption of intestinal homeostasis, E. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream.
Isolates of E. faecalis that have ended up in strain collections, mainly from clinical infection sources, appear to have acquired resistance to tetracycline and chloramphenicol in the 1950s and 1960s, followed by gentamicin and erythromycin resistance in the 1970s, then ampicillin and vancomycin resistance in the 1980s and 1990s.
Read here how to use our standards to ensure data integrity for your microbiome research.
Enterococcus faecalis is a gram positive, facultative anaerobic, non-motile, coci-shaped bacterium. It is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients. The pathogenesis of E. faecalis infection relies in part on its capacity to colonize the gut. Following disruption of intestinal homeostasis, E. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream.
Isolates of E. faecalis that have ended up in strain collections, mainly from clinical infection sources, appear to have acquired resistance to tetracycline and chloramphenicol in the 1950s and 1960s, followed by gentamicin and erythromycin resistance in the 1970s, then ampicillin and vancomycin resistance in the 1980s and 1990s.
Read here how to use our standards to ensure data integrity for your microbiome research.
Application
Inactivated bacteria are provided at >10^8 bacteria/ml concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS
Suitable for Quantitative standard for PCR, Sequencing and NGS
Features and Benefits
- Individual microbial standard for microbiomics and meta-genomics workflow
- Suitable standard for PCR, sequencing and NGS
- Improve Bioinformatics analyses
- Increases reproducibility
- Compare results lab to lab
Physical form
Liquid -The inactivated bacteria is provided at >10^8 bacteria/ml in TE buffer pH 8.0
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This Item | |||
|---|---|---|---|
| description Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | description Suitable for PCR, sequencing and NGS, 10 ng/μL | description Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | description IgG fraction of antiserum |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in ambient | shipped in dry ice | shipped in dry ice |
| technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable | technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable | technique(s) DNA extraction: suitable, PCR: suitable, DNA sequencing: suitable | technique(s) - |
| form liquid | form liquid | form liquid | form liquid |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| concentration >10^8 bacteria/ml | concentration 10 ng/μL | concentration >10^8 bacteria/ml | concentration ~1 mg/mL |
Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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What is the production strain of this product? Enterococcus faecalis ATCC 19433? What method was used to inactivate this bacterium? Heat treatment?
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