MAK313

Adipolysis Assay Kit

sufficient for 200 colorimetric or fluorometric tests

• NACRES: NA.84
• UNSPSC Code: 12161503

Properties

• application(s): pharmaceutical
• detection method: colorimetric, fluorometric
• relevant disease(s): endocrinological disorders, diabetes; obesity; cardiovascular diseases; cancer
• storage temp.: −20°C

Description

General description

Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. Adipolysis or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.

Application

Suitable for adipolysis quantification in cell culture.

Biochem/physiol Actions

This adipolysis assay kit directly measures glycerol released during adipolysis in cultured cells. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step, resulting in a colorimetric (570 nm)/fluorimetric (λex = 530/ λem = 587 nm) product, proportional to the glycerol present.

Features and Benefits

Compatible with high-throughput handling systems.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Codes: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 10 - Combustible liquids
• Flash Point (°F): 188.6 °F
• Flash Point (°C): 87 °C
• WGK: WGK 3