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MAK156

Fatty Acid Uptake Kit

sufficient for 100 fluorometric tests

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1 kit
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12 400,00 CZK
10 168,00 CZK

About This Item

UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84

10 168,00 CZK

List Price12 400,00 CZKSave 18%

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usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

storage temp.

−20°C

General description

Fatty acids are critical for many biological processes including energy metabolism and the synthesis of structural cellular components. The facilitated uptake of fatty acids, presumably through fatty acid transport proteins, is thought to be regulated at multiple levels. The dysregulation of fatty acid uptake may contribute to diseases such as obesity, type 2 diabetes, and hepatic steatosis.

Application

This kit is suitable for the measurement of fatty acid uptake in cells containing fatty acid transporters

Biochem/physiol Actions

This kit is provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. This kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate whose uptake into cells result in an increase in fluorescence intensity (λex = 485/λem = 515 nm).

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MAK174MAK225SBR00030
description

sufficient for 100 fluorometric tests

description

sufficient for 40 extractions

description

Manufactured by Nacalai Tesque, Inc.

description

-

detection method

fluorometric

detection method

-

detection method

-

detection method

-

usage

sufficient for 100 fluorometric tests

usage

sufficient for 40 extractions

usage

sufficient for 50 tests

usage

-

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

relevant disease(s)

cancer

relevant disease(s)

-

relevant disease(s)

-


Storage Class

10 - Combustible liquids



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can this kit be used in animal models to assess fatty acid uptake in vivo?

    1 answer
    1. This kit has been optimized for in vitro use with fibroblasts and adipocytes. It has not been tested for in vivo use. Based on the principle and protocol, adapting the kit for use in animal models is unlikely to be successful. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/845/984/mak156bul.pdf

      Helpful?

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