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MAK129

β-Glucosidase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Beta-Glucosidase Assay Kit

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1 kit
Please contact Customer Service for Availability
15 600,00 CZK
12 792,00 CZK

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

12 792,00 CZK

List Price15 600,00 CZKSave 18%

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usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

neurological disorders; gastrointestinal diseases; hematological disorder

storage temp.

−20°C

General description

β-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing b(1→4)-linked D-glucose residues with the release of D-glucose. β-Glucosidases are required by organisms for the consumption of cellulose. Lysozyme, a β-glucosidase present in tears, acts on the β(1→4) glucose bonds present in the peptidoglycan cell wall of Gram-negative bacteria and helps to prevent bacterial infections in the eye. Defects in β-glucosidase activity have been implicated in Gaucher′s disease and Parkinson′s disease.

Application

β-Glucosidase Activity Assay Kit has been used to measure the β-glucosidase activity of the sample.
The β-Glucosidase Activity Assay kit provides a simple and direct procedure for measuring β-glucosidase activity in biological samples, for characterizing β-glucosidase production, and for the screening of β-glucosidase modulators.

Biochem/physiol Actions

In this assay, β-glucosidase activity is determined by a reaction in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the β-glucosidase activity present. One unit of β-Glucosidse is the amount of enzyme that catalyzes the hydrolysis of 1.0 μmole substrate per minute at pH 7.0.

Features and Benefits

Compatible with high-throughput handling systems.

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MAK123MAK501MAK136
description

sufficient for 100 colorimetric tests

description

sufficient for 100 colorimetric tests

description

sufficient for 100 colorimetric or fluorometric tests

description

sufficient for 100 fluorometric tests

Gene Information

human ... GBA(2629), GBA2(57704), GBA3(57733)
mouse ... GBA(14466), GBA2(230101)
rat ... GBA(684536), GBA2(298399), GBA3(289687)

Gene Information

human ... GAA(2548)
mouse ... GAA(14387)
rat ... GAA(367562)

Gene Information

-

Gene Information

human ... MAOA(4128), MAOB(4129)
mouse ... MAOA(17161), MAOB(109731)
rat ... MAOA(29253), MAOB(25750)

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

-

usage

sufficient for 100 fluorometric tests

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric, colorimetric

detection method

fluorometric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

neurological disorders; gastrointestinal diseases; hematological disorder

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders

relevant disease(s)

endocrinological disorders, diabetes

relevant disease(s)

neurological disorders


Storage Class

12 - Non Combustible Liquids

WGK

WGK 2



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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