Product No. MAK107 comes with a "Substrate Reagent" component which is used as the substrate in this assay.
MAK107
LCAT Activity Assay Kit
Supplied by Roar Biomedical, Inc.
Synonym(s):
Lecithin:cholesterol acyltransferase activity assay kit
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About This Item
Pricing and availability is not currently available.
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usage
sufficient for 100 fluorometric tests
application(s)
pharmaceutical
detection method
fluorometric
relevant disease(s)
cardiovascular diseases
storage temp.
−20°C
Gene Information
human ... LCAT(3931)
General description
The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.
Application
Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for measuring phospholipase activity of LCAT in plasma or serum.
Principle
The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.
Kit Components Only
Product No.
Description
- Substrate Reagent .1 mL
- READ Reagent 30 mL
- LCAT Assay Buffer 20 mL
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Storage Class Code
3 - Flammable liquids
Flash Point(F)
49.5 °F
Flash Point(C)
9.7 °C
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What substrate should be used to create the standard curve for this assay?
1 answer-
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Are products MAK107 and MAK108 compatible with mouse serum?
1 answer-
Yes, but please be aware of possible sample hemolysis. Depending on the method of mouse sample collection, hemolysis may be common, which can interfere with fluorescence emission via color quenching.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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